I find that a DNA extraction does not work through some tedious protocol, but through magic! Just kidding! Thhe protocol I was using previously these past two weeks turned out to not serve me justice when looking under the UV lighting with my gels. Josh, my mentor, came up with a new protocol that turned out to result in actually exposing the DNA under the UV lighting after electrophoresis. The first protocol I used had no heating whatsoever, but in the new protocol there were two heating stages. I did however create a new DNA sample with the first protocol then used the new protocol. As before, after electrophoresis there was no DNA to be seen from the first protocol DNA sample. Below are pictures of two DNA samples from the new protocol under UV lighting after electrophoresis.
Making some new gels under a cardboard box. ;)
I am about to prepare these gels with my DNA samples for electrophoresis.
I have just taken out the gel to view under UV lighting.
The first DNA sample I made with the new protocol under UV lighting.
Mine and Josh's DNA samples from the new protocol under UV lighting.
His DNA is shown from the second to last well in the gel.
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