Tuesday, May 5, 2015

The Never-ending Droids

In the week of April 30th I was finished with my DNA extractions and began running a PCR. I used four different primers. I had five DNA extractions in total due to the five different pharaoh ant colonies. I ran all five samples separately for each primer. After the PCR, I began running a gel from each primer set. I found a trend in the polymorphic bands while looking at the gel under the UV lighting. It seemed as though colonies 1&3 had similar polymorphic bands while colonies 2,4,&5 had similar polymorphic banding with one another. Below you shall see the gels of each different primer and the DNA amplification of each colony over five lines.

Gel with DNA samples amplified with Primer 1.


Gel with DNA samples amplified with Primer 2.


Gel with DNA samples amplified with Primer 3.


Gel with DNA samples amplified with Primer 4.


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