Monday, May 4, 2015

Running Trade Federation Gels

In the week of April 16th I ran gels on the DNA samples I amplified with the PCR. It did not turn out very well;I was a bit disappointed in how the amplification turned out. I wanted to see bright,beautiful polymorphic bands, but only recieved unclear, smeared remnants of my DNA in the gel. I had used primers 1 & 2 to amplify two sets of samples. I used four samples of DNA with primer 1; one control DNA extraction and four other DNA extractions with a novel method. I did the same samples for primer 2 as well. When I ran a gel on each primer to see which primer would amplify the DNA better, I did not see good results. Below are the pictures of the gels and how they turned out. 


Gel of amplified DNA with primer 1.

Gel of amplified DNA with  primer 2.

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