This week I was able to determine what were the best methods in going about extracting DNA from the cholla spines I collected. Below are results from prickly pear cactus spines and how their banding looked after two extraction methods, but both extraction methods ran with the same Universal Rice Primers. I cannot wait to exert these methods upon the teddy-bear cholla spines collected from Dreamy Draw.
These are the gels from the first DNA extraction from the kit ran with Universal Rice Primers 1-4:
These are the gels from the first DNA extraction with InstaGene matrix ran with Universal Rice Primers 1-4:
As you can see, both DNA samples are amplified, but the better banding results from the first extraction method.
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