Thursday, November 19, 2015

Stuck in the Deserts of Tatooine

During the week of November 19th, I was still working on my extractions and began to run gels on them. I figured that if I had my samples incubate overnight in the lysis buffer the fatty membranes and proteins associated with the DNA would have broken down. I also thought I would have seen a DNA pellet form in the -20 degree isopropyl-alcohol after waiting 30 minutes, but I was not able to see anything form. After extracting most of the isopropyl-alcohol from the microcentrifuge-tube, I allowed it to air-dry underneath the hood. I then suspended the DNA in distilled water. My samples underwent electrophoresis and here is the gel:
 This gel held three samples from my three separate DNA extractions. I was not able to see any DNA under the UV light. It was not something I had hoped to see.

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