Another PCR Run with Tusken Raider Spines

This week I ran another PCR with my DNA samples with the same chloroplast primers. The first PCR run included 10 microliters of distilled water, 10 microliters DNA samples, and 20 microliters of the plant master mix that included the chloroplast primers. The only changes to the protocol was increasing the amount of water to 15 microliters and changing the amount of DNA sample to 5 microliters. The amount of plant master mix into the PCR tube stayed the same. The idea was for the primers to have a higher chance of targeting the small amount of DNA to increase amplification. The result of this PCR after electrophoresis  is presented below in this gel:

We assumed the ladder ran off a bit weird due to how the gels were made. I'll need to run another fresh gel with my PCR samples. For the mean time, I'll be working on amplifying the DNA samples with other primers in the lab that include primers 1-7.