Fortunately, I was able to conduct these DNA extractions in the biology lab and I was able to retrieve a piece of cylindropuntia bigelovii from the PC greenhouse. The next steps will be to create different reagents in order to extract the DNA from the cholla spines. I also plan on collaborating with other DNA plant extractions I have read from previouse S-STEM scholars in hopes of having some luck on extracting DNA and seeing it under the UV light after electrophoresis. I need to see polymorphic banding from cholla cacti in order to answer my question on whether chollas reproduce sexually or asexually.
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