This week I began with running a new PCR with primer 4 in order to get clear banding and making fresh gels for electrophoresis. I was able to run four gels yesterday and I looked at the results. Unfortunately, they did not come out the way I had expected. The ladder did not come out as clear as the others had and the bands were just as sloppy. I will be running new gels today in order to see if it was the total amount of time running the gels that had affected my results. I will most likely change the time of how long I want my gels to run.
Colony A Primer 4 gel
Colony B Primer 4 gel
Colony C Primer 4 gel
Colony D Primer 4 gel
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