This week I am working on making more 2% Agarose gels in order to run some with my newly amplified Ant DNA. I ran the PCR last Thursday, and I am hoping for the bands in the gels to be clear. I need the bands to be clear in order to make a concise measurement of whether certain bands are similar to one another.
I ran my gel with the amplified DNA and there were no bands! I then ran another gel with my regular DNA samples and saw polymorphic banding. I wanted to make sure I actually had DNA in my samples and wanted to know why there was no DNA showing in the gels from the PCR. I will be running another gel next week on the same amplified DNA samples. I need to figure this one out.
No comments:
Post a Comment