Monday, April 27, 2015

Amplification of the Trade Federation

The week of April 9th was one filled with new techniques. Matt helped me learn how to run a PCR! I was excited to see if the DNA samples I had would be amplified . The process was not as hard as actually doing a DNA extraction. After I was done running the PCR, I was taught how to run a gel with my amplified DNA which is different from the usual electrophoresis with non-amplified DNA. Aftter I ran my gel and looked at it under the UV lighting it seemed as though the primers had done well. I used both primers instead of just one each for my DNA samples. Below is a picture of how well both primers worked with my DNA extractions.

This is a gel containing both primers 1&2 from my first PCR.

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